A cultured approach to canine urothelial carcinoma: molecular characterization of five cell lines

Table 5 Compiled FISH and oaCGH Log2 data

	CFA1	CFA6	CFA10	CFA12	CFA13	CFA19	CFA21	CFA33	CFA36	CFA38	CFA11	p
K9TCC-Pu-An
oaCGH Log₂	−0.40	−0.50	2.94	−0.30	1.23	−0.96	−0.40	−0.60	0.60	1.20	0.00	0.49
FISH Count	2.90	3.90	10.80	3.00	8.30	1.97	2.92	3.30	8.81	7.03	3.76
FISH Log₂	−0.37	0.05	1.52	−0.33	1.14	−0.93	−0.36	−0.19	1.23	0.90	0.00
K9TCC-Pu-In
oaCGH Log₂	−0.40	−0.53	1.40	0.00	0.52	−1.00	−0.40	−0.60	0.40	0.43	0.00	0.32
FISH Count	3.40	3.73	11.00	4.07	5.62	2.50	4.10	3.00	6.87	6.77	3.79
FISH Log₂	−0.16	−0.02	1.54	0.10	0.57	−0.60	0.11	−0.34	0.86	0.84	0.00
K9TCC
oaCGH Log₂	0.13	−0.30	2.40	−1.00	0.96	−1.00	0.00	0.07	0.00	0.60	0.00	0.65
FISH Count	2.23	2.10	6.96	1.13	3.96	1.06	2.00	2.60	1.97	2.03	1.97
FISH Log₂	0.18	0.09	1.82	−0.80	1.01	−0.89	0.02	0.40	0.00	0.04	0.00
K9TCC-Pu-Mx
oaCGH Log₂	0.04	0.19	0.04	−0.20	0.90	0.00	−0.20	−0.22	1.90	1.10	0.00	0.76
FISH Count	3.80	4.80	4.30	2.88	6.61	3.90	2.50	3.57	11.97	5.71	3.88
FISH Log₂	−0.03	0.31	0.15	−0.43	0.77	0.01	−0.63	−0.12	1.63	0.56	0.00
K9TCC-Pu-Sh
oaCGH Log₂	−1.00	−1.30	1.30	−1.00	0.93	0.00	−1.80	−1.05	0.20	−1.30	0.00	1.00
FISH Count	1.93	1.25	6.33	1.47	5.34	3.14	0.64	2.00	6.19	2.13	3.68
FISH Log₂	−0.93	−1.56	0.78	−1.32	0.54	−0.23	−2.52	−0.88	0.75	−0.79	0.00

Log2 ratio values obtained from oaCGH analysis of cell lines and calculated by raw FISH data are shown. For each cell line, a Mann–Whitney U test was conducted to evaluate consistency of data between both analysis methods (p < 0.05). In all cases, the mean Log2 ratio obtained from oaCGH and FISH was not significantly different (p > 0.32 in all cases), validating oaCGH results and demonstrating the utility of oaCGH in large-scale copy number analysis. Bolded Log2 ratios demonstrate significant gains or losses (Log2 < −0.234 or Log2 > 0.2)

ISSN: 2662-9380