Resequencing of the TMF-1 (TATA Element Modulatory Factor) regulated protein (TRNP1) gene in domestic and wild canids

Table 1 Sequences of primers used for the amplification of the TRNP1 gene and transcript

Primer name	Oligonucleotide sequence (5`→3`)	Region/length amplified
Primers for gDNA template
cfTRNP1_prom_F cfTRNP1_prom_R	TGGGTCTGTTTCCTCCTCTG CGGAGTCAAGGTCGGAGTT	Proximal promoter/exon 1 c.-829 to c.135
cfTRNP1_ex1_1F cfTRNP1_ex1_2R	CGTCGGTCTTCCCCCTCG CGCTGGCCCTTCTTGAGG	Exon 1/Coding sequence c.-76 to c.*94
cfTRNP1_ex2_F cfTRNP1_ex2_R	AAGAGTTTGGGTGGGGTTGG TGCGGAATACAAGTTCTGGGT	Exon 2/3`UTR c.143 − 169 to c.802
Primers for cDNA template
cfTRNP1_839F cfTRNP1_968R	CGGGAAGAGACAGTCGGG TGTTTAAGGTTGTGGGACGTC	Partial transcript (within 3`UTR) c.97 to c.246
cfTRNP1_868F cfTRNP1_1445R	CAGTGAAGGAGGCTGCTC CTCCCTGAGAATGAGGTGCC	Partial transcript (within 3`UTR) c.126 to c.713

Positions are denoted with respect to the predicted start codon. 3’UTR: 3’untranslated region; underlined nucleotides indicate exon 1/2 splice junction; * indicates numbering from start of 3`UTR

ISSN: 2662-9380