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Table 4 Non-synonymous SNPs and frameshift mutations of canine TLRs in the TaqMan Open Array plate

From: Non-synonymous genetic variation in exonic regions of canine Toll-like receptors

Canine gene SNP Chr:bp position dbSNP ID AA Subst Previous detected a Validated?
TLR1 G/T 3:73542337 rs23585044 S29I Massive seq YES
  T/G 3:73543092 new S281A Massive seq YES
  G/A 3:73543185 new V312I Massive seq YES
  T/A 3:73544153 rs23572381 N634K1 CanFam 3.1 YES2
  T/A 3:73544153 rs23572381 N634K1 CanFam 3.1 NO
  G/A 3:73544221 rs23572380 S657N CanFam 3.1 YES2
TLR2 C/A 15:51463020 rs22410121 S46Y Massive seq YES
  A/0 15:51464076 rs8958543 A398- CanFam 3.1 YES2
  C/T 15:51464430 new S516L Massive seq YES
  C/T 15:51464700 new T606M Massive seq YES
TLR3 C/G 16:44623632 new E176D Massive seq YES
TLR4 T/C 11:71356420 rs22120766 V8A Massive seq NO
  G/C 11:71360743 rs22157966 A34P Massive seq YES
  G/A 11:71360887 new V82M Massive seq YES
  T/C 11:71364581 rs22145736 L167P Massive seq YES
  C/A 11:71364681 rs22189454 H200Q Massive seq YES
  A/G 11:71364769 rs22189456 K230E Massive seq YES
  G/A 11:71365120 new A347T Massive seq YES
  A/T 11:71365652 rs22124023 E524V Massive seq YES
  A/G 11:71365810 new T577A Massive seq YES
  G/A 11:71365888 rs22123995 E603K Massive seq YES
TLR5 G/A 38:23702193 rs24029590 G54E CanFam 3.1 NO
  0/C 38:23702251 rs9070448 -74C CanFam 3.1 YES2
  A/G 38:23702514 rs9070450 Y161C CanFam 3.1 NO
  A/C 38:23702539 new E169D Massive seq YES
  G/A 38:23702562 new S177N Massive seq YES
  G/C 38:23702640 rs9070451 R203P Massive seq YES
  T/C 38:23702684 rs9070452 W218R Massive seq NO
  C/T 38:23702837 rs9070447 R269C Massive seq YES
  G/A 38:23702918 new V296I Massive seq YES
  T/C 38:23703180 new L383S Massive seq YES
  G/A 38:23703237 new R402Q Massive seq YES
  G/A 38:23703279 new R416Q Massive seq YES
  T/0 38:23703591 rs9125247 T520- CanFam 3.1 YES2
  G/A 38:23703629 new G533S Massive seq YES
  G/A 38:23704233 new R734Q Massive seq YES
  G/T 38:23704331 new D767Y Massive seq YES
  C/T 38:23704562 new R844C Massive seq YES
  T/C 38:23704581 rs24029975 L850S Massive seq YES
  T/G 38:23704695 new F888C Massive seq YES
  G/A 38:23704718 new A896T Massive seq YES
  C/T 38:23705081 new H1017Y Massive seq YES
  G/A 38:23705090 new G1020S Massive seq YES
  G/A 38:23705178 new R1049Q Massive seq YES
  G/A 38:23705264 new A1078T Massive seq YES
TLR6 A/G 3:73521250 new Y182C Massive seq YES
  C/T 3:73522074 new L457F Massive seq YES
  G/A 3:73522242 rs23570247 D513N Massive seq YES
TLR7 C/G X:9334108 new A16G Massive seq YES2
  C/A X:9355727 new F167L Massive seq YES
  C/T X:9358423 new P1066L Massive seq YES
TLR8 T/C X:9397240 new V157A Massive seq YES
  G/A X:9397663 new R298Q Massive seq YES
  G/A X:9398094 rs24607342 G442S Massive seq YES
  G/A X:9398827 rs24607358 R686H Massive seq YES
TLR9 G/A 20:37544129 new V87I Massive seq YES
  0/A 20:37544851 rs9188882 -328A CanFam 3.1 YES2
  A/G 20:37545011 new K381E Massive seq YES
  C/A 20:37545245 new P459T Massive seq YES
  A/G 20:37546031 rs22882109 S721G Massive seq YES
  C/T 20:37546230 new P787L Massive seq ND3
  C/T 20:37546454 new R862W Massive seq YES
TLR10 C/T 3:73569402 rs23518574 T361M Massive seq YES
  A/G 3:73570094 new M592V Massive seq YES
  1. aMassive seq indicates a SNP variant detected in our cohorts. An “rs” name is indicated in dbSNP ID if the SNP is annotated in CanFam 3.1. 1SNP considered twice with a different surrender SNP in order to detect it. 2Assay has been validated technically, although not genetically because all individuals have only the reference allele. 3ND (not determined), there are incongruent results: massive sequencing showed that this SNP was present at a frequency of 0.2 in all breeds tested, whereas it has not been genotyped through TaqMan OA plate.