Homozygosity mapping and sequencing identify two genes that might contribute to pointing behavior in hunting dogs

Table 2 Chromosomal regions typed in homozygous state (H.S. score >0.8) in pointing and herding dogs

	H.S. score	Chromosomal region	Size	Genes comprised
Pointing vs. herding dogs	1.0	chr22:5,120,014-5,933,118	~1.0 Mb	ATP5H, RCBTB1, RCBTB2, PHF11, *SETDB2* , CAB39L, CDADC1, MLNR, FNDC3A, *CYSLTR2*
Pointing vs. herding dogs	1.0	chr13:11,505,788-12,451,615	~1,0 Mb	*RSPO2* , EIF3E, TTC35, EMC2, HSP90AB1, *TMEM74*

Candidate regions chosen for subsequent NGS analyses were defined as regions showing recurrent hits surpassing the 0.8 threshold using the program Homozygosity Mapper for the three analyzed file sets (see also Table S5). Primary candidate regions were narrowed down by selection of regions uniformly comprising 100% homozygosity. Candidate genes containing potential behaviorally relevant sequence variations as identified by NGS analysis are highlighted in bold.

ISSN: 2662-9380