Expression Analysis. A) Western blot analysis of skeletal muscle samples. Left panel shows detection of dystrophin by an antibody targeting the N-terminal region (ab131315). Bands are visible for full length dystrophin (* predicted weight 427 kDa) for both of the control samples, but not the DD-MD case. The right panel shows a Coomassie blue stained SDS-PAGE protein gel which was run in parallel, showing similar distribution and intensity of bands for all three samples. B) Relative levels of DMD transcript in skeletal muscle samples demonstrated by qRT-PCR. The DD-MD case shows a 2.64 fold lower expression of DMD transcript in comparison to five independent skeletal muscle controls. Error bars are based on standard deviation.