Fig. 1

oaCGH profiles of five canine UC cell lines recapitulates those seen in 31 primary tumors. Cell lines were analyzed via oaCGH at an early stage (<p16) and aberrations called based on Log₂ ratios relative to normal canine DNA (a). When compared with 31 primary tumors previously analyzed (b), aberrations were shared between the two cohorts, validating the use of canine UC cell lines in genomic studies. When clustered by oaCGH aberration profiles (c), the cell lines (astericked; TCC2 = K9TCC-Pu-An, TCC3 = K9TCC, TCC4 = K9TCC-Pu-Sh, TCC5 = K9TCC-Pu-Mx, TCC6 = K9TCC-Pu-In) clustered among the primary tumors (no asterisk) rather than appearing as an outgroup, further showing their greater similarity to the primary tumors than to one another and showing minimal effect of the culture environment on genome evolution