An autosomal recessive mutation in SCL24A4 causing enamel hypoplasia in Samoyed and its relationship to breed-wide genetic diversity

Table 1 Primers designed for SLC24A4 gene amplification and sequencing

Exon	Prod.(bp)	Forward primer 5′-3’	Reverse primer 5′-3’
1	908	CCAAATCAGGGAGCCTCAGAG	GTTCTTGCCTGGGCGTGAG
2	388	GTATCACTCCCTACAGGTGGCTCTA	TACCTTCCGTGCCTTACAGCC
3	641	GGTGGCTAATCCAGCGTTCG	GCATCATGGGAATCACGACAGT
4	529	CCGCTGCTGCTTAGAGATGC	GGAAGCACCTGTCCTCAGAAGC
5–7	1669	ACAGGGGACTCGGGCTGA	GTGCTGTGGGTTCTCTGGTCA
8	555	GTAGGCAGGTGCGTAGGGACA	AGGAGCACTGAGGAAACCATCTG
9–10	1916	GGGCTTCAGATGGCAGGATG	GGATGGCAGGTGAGAGTGAGG
11	648	TCAGGATGGGCTCAGCAAGAG	GGGGCAGGACTGGATTGAGG
12	617	GCAAACTTAGAGGGATGGTGAGG	TCAAACTACGGGTGATGTGTGG
13	506	GGGTGACATTATGGAGGTGTGTG	CCACACACCTCTGCTGAAACC
14	677	GCTGTCCTGAATCAGAACCTGG	GCTTTGTTCAATGTCACACTGGTG
15–16	1017	AGTCCTCTGTGGAGAGCAGCG	AACAATGGACGGGTCGGTG
17	599	GCAGCAGCCATGTGTCCTG	GAGGGCAGGGCTCCGAG
6 s^a		GGCTGTGCTGAAACCCTGG
16 s^a		GCACTAGGCTCTGAGAACACTGC

^a6s and 16 s are additional primers required for sequencing of the large exons 6 and 16