Fig. 2

Two-way repeated measured analysis of variance comparing leukocyte production of tumor necrosis factor (TNF)-α between blood samples incubated in calcitriol (blue lines, n = 10) and control (red lines, n = 10) with subsequent exposure to phosphate buffered saline (PBS), lipopolysaccharide (LPS), or lipoteichoic acid (LTA). The black lines represent the mean, bars the SD, and the black circles are individual dog data. There were significant between-treatment (p = 0.009) and between-pathogen associated molecular pattern (PAMP)-exposure (p < 0.001) differences. There was not a treatment-by-PAMP exposure interaction (p = 0.08)