Fig. 1

Venn diagrams comparing agglutination, spherocytosis, and DAT+ results in 120 dogs suspected to have IMHA. Microtiter plate direct antiglobulin test (MICRO) results with goat anti-canine IgG, IgM, and C3 incubated at 22°C (A) and ≥ 4 DAT+ results (B). Six of 126 cases were excluded due to strong agglutination making the evaluation of the presence of spherocytosis impossible. MICRO DAT+ : microtiter plate direct antiglobulin test method with goat anti-dog IgG, IgM, and C3 incubated at 22°C; SAT: 1:1 macroscopic saline agglutination test before washing; SPHERO: spherocytosis defined as ≥ 5 spherocytes per 100 high power field