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Table 2 Direct antiglobulin test results compared to titers and prozone effect in dogs suspected to have IMHA

From: Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs’) test

MICRO DAT

Titer

Frequency

n (%)

Prozone

n (%)

 ≥ 4 DAT+ 

n (%)

DAT-

 < 1:2

56 (44)

0

3 (4)

1:2

1 (1)

0

1 (1)

1:4

2 (2)

0

2 (3)

DAT + 

1:32

2 (20

0

1 (1)

1:64

1 (1)

0

1 (1)

1:256

4 (3)

1 (3)

4 (6)

1:512

6 (5)

3 (9)

5 (7)

1:1024

6 (5)

0

5 (7)

1:2048

48 (38)

30 (88)

45 (67)

Total Dogs n

126

34

67

  1. Titers of 1:8 and 1:16 were not seen; n: number of dogs
  2. DAT direct antiglobulin test, MICRO DAT microtiter plate DAT with goat anti-dog IgG, IgM, and C3 reagent incubated at 22°C, ≥ 4 DAT+ at least four different DAT results were positive among the five to six performed DATs
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Canine Medicine and Genetics

ISSN: 2662-9380