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Table 3 Direct antiglobulin test results with five methods in dogs suspected to have IMHA

From: Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs’) test

DAT Method

DAT-

1 DAT+ 

2 DAT+ 

3 DAT+ 

4 DAT+ 

5 DAT+ 

MICRO

35

0

1

6

13

53

GEL KIT

35

2

1

4

14

53

GEL LAB

35

7

0

1

5

53

STRIP KIT

35

6

2

5

13

53

CAPIL

35

0

0

5

11

53

Total (n = 126)

35

15

2

7

14

53

  1. Results with MICRO DAT include all reagents and temperature; results of GEL LAB and CAPIL DAT with both reagents. Any positivity with one or more reagents or temperatures was considered DAT+ 
  2. DAT Direct antiglobulin test, STRIP KIT in-clinic immunochromatographic strip kit, CAPIL microcapillary tube, MICRO microtiter plate, GEL KIT in-clinic gel minitube kit, GEL LAB neutral gel column card with added antiglobulin, FLOW was excluded because of lower number of samples tested