Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs’) test

Table 4 Comparison of Cohen’s kappa (κ) values of six direct antiglobulin test (DAT) results from 126 dogs suspected to have IMHA

DAT: direct antiglobulin test, DAT methods: STRIP KIT: in-clinic immunochromatographic strip kit; FLOW: flow cytometry (n = 69); CAPIL: microcapillary tube; MICRO: microtiter plate; GEL KIT: in-clinic gel minitube kit; GEL LAB: neutral gel column card with added antiglobulin Reagents: GAD: goat anti-dog IgG, IgM, and C3; RAD: rabbit anti-dog IgG, IgM, and C3, GAD*: FITC-marked goat anti-dog IgG (H + L) Interpretation of κ values: Groups were compared by Cohen’s kappa, κ = .37 to .82, p < .001 Green: very good agreement (≥ .81); yellow: good agreement (≥ .61); blue: moderate agreement (≥ .41); white: fair agreement (≥ .21), as per Landis and Koch [32] and adapted by Brennan and Silman [33]

ISSN: 2662-9380