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Table 5 Results of six DAT methods compared to microtiter plate DAT in 126 dogs suspected to have IMHA

From: Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs’) test

  1. DAT: Direct antiglobulin test
  2. DAT methods: STRIP: in-clinic immunochromatographic strip kit; FLOW: flow cytometry (n = 69); CAPIL: microcapillary tube; MICRO: microtiter plate; GEL KIT: in-clinic gel minitube kit; GEL LAB: neutral gel column card with added antiglobulin
  3. Reagents: GAD: goat anti-dog IgG, IgM, and C3; RAD: rabbit anti-dog IgG, IgM, and C3, GAD*: FITC-marked goat anti-dog IgG (H + L)
  4. Interpretation: > 90% (bold) represent excellent analytical sensitivity and specificity values
  5. NA: not applicable, because everything was compared to MICRO GAD 22°C
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Canine Medicine and Genetics

ISSN: 2662-9380