Comparative study of immunohematological tests with canine blood samples submitted for a direct antiglobulin (Coombs’) test

Table 6 Routine blood and immunohematological test results of 12 DAT + dogs with IMHA at time of original testing and during follow-up

Dog #	Weeks from diagnosis	Hematology					DAT
Dog #	Weeks from diagnosis	PCV (%)	Free Hb (g/dL)	Reticulo-cytosis	Aggluti-nation	Sphero-cytosis	STRIP KIT	GEL KIT	GEL LAB	MICRO	CAPIL
1	1	15/26	4.9/0.6	NA/ +	- / -	+ / +	3+/2+	4+ /4+	4+/3+	1:2048/1:2048	+ / +
2	1	21/24	NA/0.0	+ / +	- / -	+ / +	1+/ -	4+/ -	2+/ -	1:2048/ -	- / -
3	2/19	26/36/40	0.1/0.1/NA	+ / + / +	+ / - / -	+ / - / -	4+ / - / -	4+/ -	4+/ -	1:128/ -/ -	- /-/ -
4	2	37/35	1.6/1.2	- /NA	+ / -	+ / +	1+/ -	4+/4+	4+/4+	1:2048/ -	+ / -
5	4	24/40	6.0/0.7	NA/ +	- / -	+ / -	3+/1+	4+/ -	4+/4+	1:2048/1:2048	+ / -
6	4	27/37	0.2/0.2	NA/ -	+ / +	+ / -	3+/2+	4+/4+	4+/ -*	1:512/ -	- / -
7	6	16/58	0.1/0.3	+ / -	- / -	- / -	4+/ -	4+/ -	4+/ -	1:32/ -	+ / -
8	7	13/42	2.3/2.6	+ / +	+ / +	+ / +	4+/1+	4+/3+	4+/ -*	1:128/1:2048	+ / +
9	8/20	26/42/47	3.1/0.3/1.6	+ / + / -	- /-/ -	+ / - / +	2+/3+/2+	4+/4+/4+	4+/4+/2+	1:2048/1:2048/1:2048	+ / + / -*
10	10	18/53	0.4/0.1	- / -	+ / -	+ / -	2+/3+	4+/ -	4+/3+	1:2048/1:2048	+ / -
11	12/24	7/40/10	0.4/0.1/1.0	+ / - /NA	+ / - / +	+ /- / +	4+/1+/2+	4+/4+/4+	4+/2+/4+	1:2048/1:2048/1:2048	+/-* / +
12	14	33/37	0.1/0.1	+ / -	- / -	- / -	4+/3+	3+/ -	1+/ -	1:2/ -	+ / -

A few cases were tested two times during therapy, and thus two or three values are shown
The values prior, and during or after therapy are separated by separated by ‘/’
STRIP KIT: in-clinic immunochromatographic strip kit (DAT+ graded from 1+ to 4+), GEL KIT: in-clinic gel minitube kit (DAT+ graded from 1+ to 4+), GEL LAB: neutral gel column card with added antiglobulin (DAT+ graded from 1+ to 4+), MICRO: microtiter plate (titers equal or below to 1:4 were considered DAT-), CAPIL: microcapillary tube, Spherocytosis defined as ≥ 5 spherocytes per 100 × objective
GEL LAB, MICRO and CAPIL tested with rabbit anti-dog IgG, IgM, and C3 (RAD) and goat anti-dog IgG, IgM, and C3 (GAD) reagents
NA: not available, - : negative, + : positive, *: positive with RAD

ISSN: 2662-9380