Fig. 4
From: A cultured approach to canine urothelial carcinoma: molecular characterization of five cell lines
Gene expression arrays, qRT-PCR, and oaCGH results support one another and suggest a role of genomic copy number on mRNA levels. Seven differentially expressed genes located in copy number-aberrant regions (see Table 3) were selected for qRT-PCR analysis to validate microarray results. Correlation was very strong (r = 0.96) between the two methods and direction of fold change was in agreement for all genes, confirming the gene’s differential expression is true. In addition, comparison of qRT-PCR and oaCGH Log2 ratios revealed a strong correlation (r = 0.76), demonstrating the impact of genomic gene dosage on mRNA levels