A cultured approach to canine urothelial carcinoma: molecular characterization of five cell lines

Table 2 Regions chosen for FISH validation of oaCGH

BAC address	Region probed	Fluorochrome	oaCGH-called aberration
326 N03	chr1:35846825-36021610	Red	Loss
307I06	chr6:49193262-49385361	Green	Loss
326H08	chr10:13936488-14128322	Cy5	Amplification
126 F01	chr12:52174704-52401789	Gold	Loss
186 J06	chr13:38344516-38552798	Aqua	Gain
332 N02	chr19:31345273-31534341	Red	Loss
313D22	chr21:5212350-5387435	Green	Loss
307D14	ch33:5409680-5589439	Red	Loss
199 F16	chr36:7057670-7276062	Cy5	Amplification
328D10	chr38:25164963-25365710	Gold	Amplification
326 L05	chr11:20496757-20706739	Aqua	Neutral

Ten BAC clones were selected to probe regions shown to be highly aberrant in the cell lines (>50 % penetrant). A copy number neutral region on CFA11 was chosen as an internal control to ensure correct interpretation of relative locus copy number. The BAC address, genomic location (CanFam2.0 assembly), and associated fluorochrome for each probe are shown

ISSN: 2662-9380