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Table 3 Copy number aberrant genes analyzed by qRT-PCR

From: A cultured approach to canine urothelial carcinoma: molecular characterization of five cell lines

Gene Location Forward primer Reverse primer
RNPC3 chr6:50072550-50098045 5′-GGGGCGACCGGCCCTTCTA-3′ 5′-GACAGGACGCGCACCGACTG-3′
MDM2 chr10:13920606-13946580 5′-ACGGCAGAGAAAGCGCCACAAA-3′ 5′-GGCGTCCCTGTTGACTCACTG-3′
EIF2C2 chr13:38222456-38271623 5′-CAAAGGCAGTCCAGGTTCAT-3′ 5′-GGGCATCTGTTGGTCTGAGT-3′
RALB chr19:32842484-32862087 5′-GTGTTCTTGCTCTCCCCAAC-3′ 5′-TCCAAAACCTCCCAACAAAG-3′
CLNS1A chr21:24140052-24163815 5′-CCTGTGTCTCCGCGCTCCCTG-3′ 5′-GCCTCGGTCTCGGGCTGCTG-3′
STX19 chr33:4727847-4728760 5′-CTGCTATGTTCCGCCAATTT-3′ 5′-GCACTTCTTTTCCAGCAACC-3′
TAGLN2 chr38:25240217-25241796 5′-TGCGGACCTGGAGCAGATCCTG-3′ 5′-ACACAGCACCGTGCCATCCT-3′
RPL32 chr18:10198892-10204028 5′-ATGCCCAACATTGGTTATGG-3′ 5′-ATGCCCAACATTGGTTATGG-3′
  1. The mRNA levels of genes located within seven regions of copy number aberration were evaluated by qRT-PCR. The targeted gene, genomic location (CanFam2.0 assembly), and primer pair utilized are shown