A cultured approach to canine urothelial carcinoma: molecular characterization of five cell lines

Table 6 Gene expression microarray results

Gene	Location	Lines differentially expressing	Average fold change	Gene	Location	Lines with relative fold change ≥2 or ≤ −2	Average fold change
CES1 ^a	chr2	ALL	−633.5	BIRC5 ^a	chr9	In, An, Mx, K9TCC	+3.6
HNMT	chr19	In, Sh, Mx, K9TCC	−29.6	RAF1 ^a	chr20	ALL	+4.1
DBC1 ^a	chr11	ALL	−13.6	CLU ^a	chr25	ALL	+5.5
CDKN2B ^a	chr11	ALL	−11.8	CCNG2 ^a	chr32	ALL	+6.3
FHIT ^a	chr20	ALL	−11.5	MDM2 ^a	chr10	In, Sh, An, K9TCC	+6.4
CYP2C19 ^a	chr28	ALL	−8.1	MYC ^a	chr13	ALL	+8.5
BCL2 ^a	chr1	ALL	−6.6	LAMB3 ^a	chr7	ALL	+12.4
SRPX ^a	chrX	In, An, Mx, K9TCC	−6.1	TGFBR2 ^a	chr23	ALL	+12.6
FMO5 ^a	chr17	ALL	−4.9	CCND2 ^a	chr27	ALL	+14.2
SPARC ^a	chr4	ALL	−4.5	AURKA ^a	chr24	ALL	+22.2
PTEN ^a	chr26	ALL	−4	TAGLN2 ^a	chr38	ALL	+25.3
BMP7 ^a	chr24	An, Mx, K9TCC	−3.4	PTTG1 ^a	chr4	ALL	+57.5
DAPK1 ^a	chr1	An, Mx	−2.8	CDK1 ^a	chr4	ALL	+72.5
VHL ^a	chr20	ALL	−2.1	PTGS2 ^a	chr7	ALL	+76.9

Numerous genes were dysregulated in the cell lines when compared to the expression profile of healthy canine urothelium. The genes listed were selected based on their magnitude of fold change, oncogenic potential, and relevance in human UC (see superscipt letter a). The specific cell lines differentially expressing each gene are listed (fold change ≥2 or ≤ −2 relative to the mean expression of two healthy urothelial samples)

ISSN: 2662-9380